منابع مشابه
"Stat" multiplex polymerase chain reaction: its role in clinical microbiology.
In the laboratory, the department most affected by the introduction of molecular diagnostic tools has been the clinical microbiology laboratory. For organisms such as Clostridium difficile, polymerase chain reaction (PCR) is the gold standard for diagnosis. Incorporation of molecular diagnostic techniques into microbiology laboratories of differing sizes has been hastened by the development of ...
متن کاملRfbo157 by Multiplex Polymerase Chain Reaction
A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...
متن کاملMultiplex polymerase chain reaction diagnostics of bed bug (Hemiptera: Cimicidae).
Cimex lectularius L. (Hemiptera: Cimicidae) is a widespread blood feeding pest of humans around the world, including North America, and has recently undergone a resurgence. A molecular diagnostic technique applying multiplex polymerase chain reaction (PCR) was developed to distinguish bed bug eggs, leg fragments, and degraded samples from other arthropods that frequently occur in human dwelling...
متن کاملIdentification of Dermatophytes Using Multiplex Polymerase Chain Reaction
BACKGROUND Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. OBJECTIVE To assess the value of multiplex ...
متن کاملStreptococcus pneumoniae Type Determination by Multiplex Polymerase Chain Reaction
The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77 isolates from clinical specimens collected from children at Seoul National University Children's Hospital from 2006 to 2010. These strains were selected as they represented 26 different serotypes previ...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2013
ISSN: 1943-7722,0002-9173
DOI: 10.1309/ajcpequhcjba8c6t